3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 98%, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Thiazolyl blue tetrazolium bromide, MTT, 2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazol-3-ium bromide

M-Clarity™ quality level = MQ200

M2128-5G M2128-100MG M2128-10G M2128-1G M2128-250MG M2128-500MG
M2128-5GEA 534 GBP
M2128-5G M2128-100MG M2128-10G M2128-1G M2128-250MG M2128-500MG
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 98%, Sigma-Aldrich®
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.


  • Thiazolyl Blue Tetrazolium Bromide has been used for the following assays:
  • Proliferation assay
  • TNF (tumor necrosis factor) bioassay

Formula: C₁₈H₁₆N₅BrS
MW: 414,33 g/mol
Melting Pt: (dec., lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00011964
CAS Number: 298-93-1
EINECS: 206-069-5
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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Purity (HPLC) ≥98.0%
Solubility (Turbidity), c = 5 mg/ml (0.5%); Water Clear
Solubility (Colour) Yellow to Very Dark Yellow and Yellow-Green
Recommended Retest Period 5 years

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