Wright's stain used as a blood stain, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Eosin Methylene blue solution according to Wright, Wright's eosin methylene blue

M-Clarity™ quality level = MQ200

W0625-100G W0625-10G W0625-25G
W0625-100GEA 236 GBP
W0625-100G W0625-10G W0625-25G
Wright's stain used as a blood stain, Sigma-Aldrich®
Wright's stain

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.


  • Wright's Stain is mainly used for the staining of peripheral blood smears and bone marrow smears
  • It is also used for G-banding and helps in the analysis of poorly spread metaphases
  • It has been used to study the cytogenetics of human hematopoietic cells
  • It has also been employed in the karyotyping of human embryonic stem cells
  • It is also used in the staining of chromosomes
  • It is used for the exudate analysis of white blood cell concentration
  • Reported to be superior to May-Grunwald Stain in blood staining


The purified dyes in Wright's Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.


Prepared using polychromed (oxidised) methylene blue and eosin Y.

Density: 0,8 g/cm³ (20 °C)
Storage Temperature: Room Temperature
MDL Number: MFCD00082143
CAS Number: 68988-92-1
EINECS: 273-541-5

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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Solubility (Colour)
c = 100 mg/100 ml; Methanol
Purple to Very Dark Purple to Very Dark Blue and Black
Solubility (Turbidity) Clear to Turbid
Assay (HPLC)
Impurity: Azure C
≤4 %
Suitability
Suitable for use in blood staining
Suitable

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