Wright's stain used as a blood stain, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Warning
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Synonyms:
Eosin Methylene blue solution according to Wright, Wright's eosin methylene blue
M-Clarity™ quality level = MQ200
Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.
- Wright's Stain is mainly used for the staining of peripheral blood smears and bone marrow smears
- It is also used for G-banding and helps in the analysis of poorly spread metaphases
- It has been used to study the cytogenetics of human hematopoietic cells
- It has also been employed in the karyotyping of human embryonic stem cells
- It is also used in the staining of chromosomes
- It is used for the exudate analysis of white blood cell concentration
- Reported to be superior to May-Grunwald Stain in blood staining
The purified dyes in Wright's Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.
Prepared using polychromed (oxidised) methylene blue and eosin Y.
Density:
0,8 g/cm³ (20 °C) Storage Temperature: Room Temperature |
MDL Number:
MFCD00082143 CAS Number: 68988-92-1 EINECS: 273-541-5 |
Specification Test Results
Appearance (Form) | Powder |
Infrared spectrum | Conforms to Structure |
Solubility (Colour) c = 100 mg/100 ml; Methanol |
Purple to Very Dark Purple to Very Dark Blue and Black |
Solubility (Turbidity) | Clear to Turbid |
Assay (HPLC) Impurity: Azure C |
≤4 % |
Suitability Suitable for use in blood staining |
Suitable |
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