Leishman's eosin-methylene blue for histology, stain, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Eosin and methylene blue Leishman, Eosin-Methylene blue dye Leishman, Leishman's stain, Leishman stain, Leishman's stain (eosin methylene blue compound)

M-Clarity™ quality level = MQ100

L6254-100G L6254-25G
L6254-100GEA 256 GBP
L6254-100G L6254-25G
Leishman's eosin-methylene blue for histology, stain, Sigma-Aldrich®
Leishman's Eosin-Methylene blue

Leishman stain is a Romanowsky-type dye, similar to Wright stain. Leishman stain is used in hematology and tropical medicine to differentiate and identify leukocytes, malarial parasites, and trypanosomes. It is also known as eosin-polychrome methylene blue.


Leishman′s stain has been used for staining blood smears to differentiate blood corpuscles, malarial parasites, trypanosomers, etc. It may be used as an alternative to Giemsa. Leishman’s stain has been used for the identification of macrophage nuclei in cells.It has also been used in hematology study.

Storage Temperature: Room Temperature
MDL Number: MFCD00131498
CAS Number: 12627-53-1
EINECS: 235-732-1

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Specification Test Results

Appearance (Colour) Dark Green to Very Dark Green
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Solubility (Colour), c = 1 mg/ml; Methanol Dark Blue to Very Dark Blue
Recommended Retest Period 4 Years

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