Folin-Ciocalteu's reagent 1,9 - 2,1 N for analysis of phenols, suitable for determination of total protein by Lowry method, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Danger
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Synonyms:
Folin & Ciocalteu’s phenol reagent
M-Clarity™ quality level = MQ200
Folin & Ciocalteu's phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750 nm) or shoulder (660 nm) are used to quantitate protein concentrations between 1 - 100 mg/ml while absorbance at 550 nm is used to quantitate higher protein concentrations.
- Folin & Ciocalteu's phenol reagent has been used for the estimation of total phenolic content in samples.
Many modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay. Folin & Ciocalteu's phenol reagent can also be used as a spray reagent in chromatographic procedures.
In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.
Density:
1,22 g/cm³ (20 °C) Storage Temperature: Room Temperature |
MDL Number:
MFCD00132625 UN: 3264 ADR: 8,III |
Specification Test Results
Appearance (Turbidity) | Clear |
Appearance (Colour) | Faint Yellow to Very Dark Yellow and Faint Green-Yellow to Very Dark Green-Yellow |
Appearance (Form) | Solution |
Concentration | 1.9 - 2.1 N |
Suitability Suitable for Use in Lowry Protein Determination |
Suitable |
Recommended Retest Period | 4 years |
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