Light green SF yellowish, powder or crystals, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: CI 42095Sodium 3-((-ethyl(-4-((4-(ethyl(3-sulphonatobenzyl)amino)phenyl)(4-sulphonatophenyl)methylene)cyclohexa-2,5-dien-1-ylidene)ammonio)methyl)benzenesulphonateDihydrogen (ethyl)[4-[p-[ethyl(m-sulphonatobenzyl)amino]-p'-sulphonatobenzhydrylene]cyclohexa-2,5-dien-1-ylidene](m-sulphonatobenzyl)ammonium disodium saltacid Green FLight Green 2G, S or 2GNacid Green 5Fast acid Green NCN DcL-30Food Green n°2

M-Clarity™ quality level = MQ100

L5382-25GEA 225 GBP
L5382-25G L5382-100G L5382-10G
Light green SF yellowish, powder or crystals, Sigma-Aldrich®
Light Green SF Yellowish

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.


Light green SF is generally used as a cytoplasmic counterstain with nuclear stains like safranine and Feulgen stain. In histopatology, it is used for collagen fiber staining in Masson′s trichrome, Papanicolaou cytological polychrome stains, and Twort stain for microorganisms in tissue sections. It is a constituent of polychrome staining method used for differentiating secretory cells of the pituitary gland. Light green SF also stains the cellulose cell walls of plant cells.

Formula: C₃₇H₃₄N₂Na₂O₉S₃
MW: 792,86 g/mol
Melting Pt: 288 °C (dec., lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00012121
CAS Number: 5141-20-8
EINECS: 225-906-5
Merck Index: 13,05506

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Specification Test Results

Appearance (Colour) Dark Red to Purple
Appearance (Form) Powder or Crystals
Nitrogen 2.1 - 3.5 %
Sodium (Na) ≥5%
Solubility (Colour) Dark Blue-Green to Very Dark Blue-Green to Very Dark Green and Dark Brown
Solubility (Turbidity), 10 mg/ml, H₂O Clear
E1% ≥600
Wavelength in H₂O 631 - 634 nm

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