Methyl blue, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
Synonyms:
Aniline blue sodium salt, Acid Blue 93, [[4-[bis[4-[(sulphophenyl)amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]amino]-benzenesulphonic acid sodium salt (1:2), Poirriers blue, Cotton blue, Water blue, C.I. 42780, Aniline blue, water soluble, Disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate
M-Clarity™ quality level = MQ200
Methyl blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.
- Methyl blue has been used to stain leaf disks for callose visualisation within the cells
- It has been used to incubate cells for 4',6-diamidino-2-phenylindole (DAPI)/septa staining
- Used as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
- Used in Mann's stain as it involves a methyl blue-eosin mixture
Formula:
C₃₇H₂₇N₃Na₂O₉S₃ MW: 799,81 g/mol Flash Pt: >300 °C - Pensky-Martens (closed cup) Storage Temperature: Ambient |
MDL Number:
MFCD00058509 CAS Number: 28983-56-4 EINECS: 249-352-9 Merck Index: 13,06055 |
Specification Test Results
Appearance (colour) | Purple to very dark purple |
Appearance (form) | Powder or crystals |
Thin layer chromatogr. | Corresponds to standard |
Solubility (colour) | 0.01 g in 10 ml water |
Solubility (method) | 0.01 g in 10 ml water |
pH-transition | 14.0 |
Colour | Pink |
Wavelength (1) (UV) | 595.0 - 605.0 nm |
Extinction (1%/1 cm) (1) (UV) | ≥400 |
Wavelength (2) (UV) | 310.0 - 320.0 nm |
Extinction (1%/1 cm) (2) (UV) | ≥200 |
Solvent (UV) | HCl 0.005 m |
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