Methyl blue, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Aniline blue sodium salt, Acid Blue 93, [[4-[bis[4-[(sulphophenyl)amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]amino]-benzenesulphonic acid sodium salt (1:2), Poirriers blue, Cotton blue, Water blue, C.I. 42780, Aniline blue, water soluble, Disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate

M-Clarity™ quality level = MQ200

95290-100G 95290-25G 95290-5G
95290-100GEA 262 GBP
95290-100G 95290-25G 95290-5G
Methyl blue, Sigma-Aldrich®
Methyl blue

Methyl blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.


  • Methyl blue has been used to stain leaf disks for callose visualisation within the cells
  • It has been used to incubate cells for 4',6-diamidino-2-phenylindole (DAPI)/septa staining
  • Used as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • Used in Mann's stain as it involves a methyl blue-eosin mixture

Formula: C₃₇H₂₇N₃Na₂O₉S₃
MW: 799,81 g/mol
Flash Pt: >300 °C - Pensky-Martens (closed cup)
Storage Temperature: Ambient
MDL Number: MFCD00058509
CAS Number: 28983-56-4
EINECS: 249-352-9
Merck Index: 13,06055
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Specification Test Results

Appearance (colour) Purple to very dark purple
Appearance (form) Powder or crystals
Thin layer chromatogr. Corresponds to standard
Solubility (colour) 0.01 g in 10 ml water
Solubility (method) 0.01 g in 10 ml water
pH-transition 14.0
Colour Pink
Wavelength (1) (UV) 595.0 - 605.0 nm
Extinction (1%/1 cm) (1) (UV) ≥400
Wavelength (2) (UV) 310.0 - 320.0 nm
Extinction (1%/1 cm) (2) (UV) ≥200
Solvent (UV) HCl 0.005 m

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