Coomassie brilliant blue G-250, pure, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Brilliant blue G-250, Serva Blue G, Coomassie Blue G 250, Xylene Brilliant Cyanine G, Brillant blue G, Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]-2'-methylbenzhydrylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt, C.I. 42655, Acid Blue 90, Sodium 3-(((4-((4-((4-ethoxyphenyl)amino)phenyl)(4-(ethyl(3-sulphonatobenzyl)iminio)-2-methylcyclohexa-2,5-dien-1-ylidene)methyl)-3-methylphenyl)(ethyl)amino)methyl)benzenesulphonate, Brilliant indocyanine G, Brilliant Blue G

M-Clarity™ quality level = MQ200

B0770-100G B0770-25G B0770-5G
B0770-100GEA 707 GBP
B0770-100G B0770-25G B0770-5G
Coomassie brilliant blue G-250, pure, Sigma-Aldrich®
Coomassie brilliant blue G-250

Brilliant Blue G has been used for determining the urine protein concentration. It has been used for the protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used as a P2X7R (purinergic receptor) antagonist.


Brilliant Blue G is used for staining protein(s) in polyacrylamide gels. It is also a P2X7 purinergic receptor antagonist. The receptor is mainly involved in neuroinflammatory events. In human SOD1G93A amyotrophic lateral sclerosis mouse, administration of brilliant blue G reduces body weight loss. However, it does not affect survival or motor coordination in transgenic mice. P2X7 purinergic receptor antagonist.

Formula: C₄₇H₄₈N₃NaO₇S₂
MW: 854,04 g/mol
Density: >1 g/cm³ (20 °C)
Storage Temperature: Room Temperature
MDL Number: MFCD00078482
CAS Number: 6104-58-1
EINECS: 228-058-4
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Specification Test Results

Appearance (Form) Powder
Solubility (Colour) Dark Blue to Very Dark Blue
Solubility (Form), 1 mg/ml, H₂O Solution
Suitability, Suitable for use as a reagent in protein determinations Suitable
Purity (HPLC) ≥80%
Recommended Retest Period 4 years

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