Wright's stain, certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
Warning

Synonyms: Eosin Methylene blue solution according to Wright, Wright's eosin methylene blue

M-Clarity™ quality level = MQ200

861375-100G 861375-25G 861375-SPEC 861375-VAR
861375-100GEA 419 GBP
861375-100G 861375-25G 861375-SPEC 861375-VAR
Wright's stain, certified by the Biological Stain Commission, Sigma-Aldrich®
Wright's stain

Wright stain, a modified romanowsky stain, is intended for differential staining of the cellular elements of blood. It comprises polychrome methylene blue and eosin B. Upon staining blood films with wright stain, the white blood cell nucleus and cytoplasm display characteristic blue or pink hues.


  • The purified dyes in wright stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses
  • Reported to be superior to May-Grunwald stain in blood staining
  • Certified by the biological stain commission


Wright stain is intended for use in staining peripheral blood smears and bone marrow smears. It has been used to study the cytogenetics of human hematopoietic cells. It has also been employed in the karyotyping of human embryonic stem cells. It is also used for G-banding and helps in the analysis of poorly spread metaphases. Reported to be superior to May-Grunwald stain in blood staining.

Density: 0,8 g/cm³ (20 °C)
Storage Temperature: Room Temperature
MDL Number: MFCD00082143
CAS Number: 68988-92-1
EINECS: 273-541-5

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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Biological Stain Commission
Commission Certified Number
Pass
Solubility (Turbidity)
c = 1 mg/ml, Methanol
Clear to Turbid
Solubility (Colour) Purple to Very Dark Purple to Very Dark Blue and Black
Assay (HPLC)
Impurity: Azure C
≤4%

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