Tetrazolium blue chloride for colorimetric determination of reducing compounds, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
Synonyms:
Blue Tetrazolium, 3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis(2,5-diphenyl-2H-tetrazolium chloride), BTC, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), Blue Tetrazolium chloride
M-Clarity™ quality level = MQ200
Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.
Formula:
C₄₀H₃₂Cl₂N₈O₂ MW: 727,65 g/mol Melting Pt: 255 °C (dec., lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00040933 CAS Number: 1871-22-3 EINECS: 217-488-8 Merck Index: 12,09380 |
Specification Test Results
Appearance (Colour) | Yellow |
Appearance (Form) | Powder |
Solubility (Colour) | Yellow to Yellow-Green |
Solubility (Turbidity) 50 mg/mL, MeOH |
Clear |
Infrared spectrum | Conforms to Structure |
Carbon | 63.4 - 67.4 % |
Nitrogen | 14.9 - 15.9 % |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...