Tetrazolium blue chloride for colorimetric determination of reducing compounds, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Blue Tetrazolium, 3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis(2,5-diphenyl-2H-tetrazolium chloride), BTC, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), Blue Tetrazolium chloride

M-Clarity™ quality level = MQ200

T4375-10G T4375-1G T4375-25G T4375-5G
T4375-10GEA 349 GBP
T4375-10G T4375-1G T4375-25G T4375-5G
Tetrazolium blue chloride for colorimetric determination of reducing compounds, Sigma-Aldrich®
Tetrazolium blue chloride

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

Formula: C₄₀H₃₂Cl₂N₈O₂
MW: 727,65 g/mol
Melting Pt: 255 °C (dec., lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00040933
CAS Number: 1871-22-3
EINECS: 217-488-8
Merck Index: 12,09380
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Specification Test Results

Appearance (Colour) Yellow
Appearance (Form) Powder
Solubility (Colour) Yellow to Yellow-Green
Solubility (Turbidity)
50 mg/mL, MeOH
Clear
Infrared spectrum Conforms to Structure
Carbon 63.4 - 67.4 %
Nitrogen 14.9 - 15.9 %

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