New methylene Blue N ~70% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Basic Blue 24, Methylene Blue N

556416-10G 556416-1G
556416-10GEA 381 GBP
556416-10G 556416-1G
New methylene Blue N ~70% (dye content), Sigma-Aldrich®
New methylene Blue N

New methylene blue N has been used as a photosensitizer for the photodynamic inactivation of Candida albicans.


  • Solvent soluble cationic thiazine dye


New methylene blue N can bind, neutralise and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N helps in counting of reticulocytes (erythrocytes containing RNA).

Formula: C₁₈H₂₂ClN₃S
MW: 347,91 g/mol
Melting Pt: 190 °C (dec., lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00135878
CAS Number: 1934-16-3
EINECS: 217-696-9

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Specification Test Results

Appearance (Colour): Dark Green to Dark Brown Conforms to Requirements
Appearance (Form): Powder, Crystals and/or Chunks Conforms to Requirements
Infrared spectrum Conforms to Structure
Wavelength, In Water 626 - 634 nm
Extinction Coefficient ≥30500
Wavelength 586 - 594 nm
Carbon 42.2 - 82.0%
Nitrogen 8.2 - 15.9%
Zinc by ICP Assay ≤100 ppm

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