Rhodamine 123 fluorescent dye, mitochondria dye

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: 3,6-Diamino-9-(2-(methoxycarbonyl)phenyl xanthylium chloride, 3,6-Diamino-9-(2-(methoxycarbonyl)phenyl)xanthylium chloride, 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

M-Clarity™ quality level = MQ100

R8004-100MG R8004-25MG R8004-5MG
R8004-100MGEA 384 GBP
R8004-100MG R8004-25MG R8004-5MG
Rhodamine 123 fluorescent dye, mitochondria dye
Rhodamine 123

Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential.


Rhodamine 123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for P-glycoprotein (Pgp). It is part of the rhodamine family of flurone dyes. The dye is mostly used to assess mitochondrial bioenergetics in living cells. Functional assays for multi-drug resistance (MDR) proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of multi-drug resistance protein-1 (MRP1).


Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Formula: C₂₁H₁₇ClN₂O₃
MW: 380,82 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00012664
CAS Number: 62669-70-9
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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Carbon (dry basis) 62.2 - 70.2 %
Nitrogen (dry basis) 6.9 - 7.8 %
Water (by Karl Fischer) ≤10.0 %
Solubility (Turbidity)
1mg/ml in Ethanol
Clear to Slightly Hazy
Solubility (Colour) Conforms to Requirements
Yellow-Orange
Recommended Retest Period 4 Years

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