6-Aminofluorescein (isomer II), Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
Synonyms:
Fluoresceinamine, 6-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one, 6-Amino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
M-Clarity™ quality level = MQ200
6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed 'buckysomes'. 6-aminofluorescein dissolved in 0,1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110 °C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein. 6-Aminofluorescein has been used to determine its ability to potentiate the antiviral activity of poly r (A-U).
Caution: Do not confuse with fluorescamine.
Formula:
C₂₀H₁₃NO₅ MW: 347,33 g/mol Melting Pt: 285 °C (dec., lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00005051 CAS Number: 51649-83-3 EINECS: 257-334-7 |
Specification Test Results
Appearance (Colour) | Yellow to Orange |
Appearance (Form) | Powder |
Infrared spectrum | Conforms to Structure |
Carbon | 65.4 - 72.9% |
Nitrogen | 3.7 - 4.3% |
Water (by Karl Fischer) | ≤5.0% |
Purity (HPLC) | ≥90.0% |
Solubility (Turbidity), c = 1 mg/ml (0.1%), Methanol | Clear |
Solubility (Colour), Dark Yellow to Orange | Conforms to Requirements |
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