6-Aminofluorescein (isomer II), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Fluoresceinamine, 6-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one, 6-Amino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one

M-Clarity™ quality level = MQ200

201634-1G 201634-250MG
201634-1GEA 120 GBP
201634-1G 201634-250MG
6-Aminofluorescein (isomer II), Sigma-Aldrich®
6-Aminofluorescein (isomer II)

6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed 'buckysomes'. 6-aminofluorescein dissolved in 0,1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110 °C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein. 6-Aminofluorescein has been used to determine its ability to potentiate the antiviral activity of poly r (A-U).


Caution: Do not confuse with fluorescamine.

Formula: C₂₀H₁₃NO₅
MW: 347,33 g/mol
Melting Pt: 285 °C (dec., lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00005051
CAS Number: 51649-83-3
EINECS: 257-334-7
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Specification Test Results

Appearance (Colour) Yellow to Orange
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Carbon 65.4 - 72.9%
Nitrogen 3.7 - 4.3%
Water (by Karl Fischer) ≤5.0%
Purity (HPLC) ≥90.0%
Solubility (Turbidity), c = 1 mg/ml (0.1%), Methanol Clear
Solubility (Colour), Dark Yellow to Orange Conforms to Requirements

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