Bromothymol blue sodium salt in ACS Reagent grade water, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Sodium α-(3-bromo-5-isopropyl-4-oxo-2-methyl-2,5-cyclohexadienylidene)-2-(3-bromo-4-hydroxy-5-isopropyl-2-methylphenyl)toluenesulphonate, Bromothymol blue water soluble, Sodium 2-bromo-4-(3-(3-bromo-4-hydroxy-5-isopropyl-2-methylphenyl)-1,1-dioxido-3H-benzo[c][1,2]oxathiol-3-yl)-6-isopropyl-3-methylphenolate

M-Clarity™ quality level = MQ200

114421-25G 114421-5G
114421-25GEA 113 GBP
114421-25G 114421-5G
Bromothymol blue sodium salt in ACS Reagent grade water, Sigma-Aldrich®
Bromothymol blue sodium salt

Bromothymol blue has been used for freshwater pH measurements over a broad range of buffer intensities.


  • pH-indicator
  • Used for carbonic anhydrase assay due to the conversion of carbon dioxide to bicarbonate
  • Used as a diagnostic assay manufacturing, hematology, histology

Formula: C₂₇H₂₇Br₂NaO₅S
MW: 646,37 g/mol
Melting Pt: >200 °C
Storage Temperature: Ambient
MDL Number: MFCD00077263
CAS Number: 34722-90-2
EINECS: 252-169-7
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Specification Test Results

Appearance (Form) Powder or Crystals
Infrared spectrum Conforms to Structure
Wavelength c = 0.005 g/L; 0.1 N NaOH 614 - 618 nm
Extinction Coefficient ≥13000
Wavelength 305 - 309 nm
Visual Transition Interval Yellow to Yellow-Green (pH 6.0) Pass
Visual Transition Interval Green (pH 6.7) Pass
Visual Transition Interval Blue (pH 7.6) Pass
Clarity of Solution, c = 0.1%; Water, Clear with only a faint trace of turbidity or insoluble matter Clear to Very Slightly Hazy

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