Neutral red 90% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: CI 50040, N2,N2,7-Trimethylphenazine-2,8-diamine hydrochloride, 3-Amino-7-dimethylamino-2-methylphenazine, N8,N8,3-Trimethyl-2,8-phenazinediamine monohydrochloride, Toluylene red, Basic Red 5

M-Clarity™ quality level = MQ200

N7005-25G N7005-5G N7005-1G
N7005-25GEA 664 GBP
N7005-25G N7005-5G N7005-1G
Neutral red 90% (dye content), Sigma-Aldrich®
Neutral red

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.


Neutral red has multiple staining applications that include its use as a nuclear stain, counterstain in the Gram procedure, either alone, or as a component of the Twort stain (a fast green-neutral red mixture). Neutral red staining is also a viability assessment method used for the detection of viable yeast cells in dandruff samples and of mussel larvae. Neutral red is also recommended for use in viability assessment as it is selectively absorbed by lysosomes and other acidic organelles in cells. It is also used for supravital staining of living blood cells. Useful as an indicator for preparing neutral red paper, and as a biological stain. Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Formula: C₁₅H₁₇ClN₄
MW: 288,78 g/mol
Melting Pt: 290 °C (dec., lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00012651
CAS Number: 553-24-2
EINECS: 209-035-8
Merck Index: 12,06571
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Specification Test Results

Appearance (Colour) Brown to Black may have red or green sheen
Appearance (Form) Powder
Solubility (Colour), 10 mg/ml, H₂O Dark Red
Infrared spectrum Conforms to Structure
Dye Content (Calculated based on UV spectral assay) ≥90%
Recommended Retest Period 4 years

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