RPMI 1640 (1X), Cell Culture Media, MP Biomedicals

Supplier: MP Biomedicals

091629149 091646454
ICNA091629149EA 71.7 GBP
ICNA091629149 ICNA091646454
RPMI 1640 (1X), Cell Culture Media, MP Biomedicals
Cell Culture Media

RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.


  • Originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI)
  • Widely used in fusion protocols and in the growth of hybrid cells
  • Used with a serum supplement, to support several cell lines in the absence of serum


It was based on the RPMI 1630 line of media which utilised a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.


The Dutch Modification of RPMI 1640 is also available through MP, contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

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