cDNA synthesis, qScript™ Ultra SuperMix
Supplier: Quantabio
Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.
- Enhanced thermostability – Engineered for RT activity up to 65 °C, overcoming challenging secondary structures
- Superior speed – 10 minute reaction time with a total protocol time of 13 minutes
- Maximum yield and sensitivity – Wide dynamic range with no loss in cDNA abundance linearity from total RNA 2,5 μg to 1 pg
- Ultimate inhibitor resistance – Overcome a wide array of PCR inhibitors (salt, heparin, melanin, etc.)
- Balanced coverage of long transcripts – Unbiased representation of the transcriptome in cDNA product
- Ease of use – Single tube mix with all required components for cDNA synthesis except RNA template
qScript Ultra SuperMix is a highly stabilized, efficient and easy-to-use single tube master mix for the synthesis of first-strand cDNA to reverse transcribe RNA to cDNA. A key component is a novel, state-of-the-art, RNase H deficient reverse transcriptase that was engineered for improved thermostability, velocity, processivity, and resistance to many common inhibitors. qScript Ultra SuperMix contains all required components for first-strand cDNA synthesis except RNA template and is directly compatible with downstream 2-step RT-qPCR or RT-PCR procedures.
qScript Ultra SuperMix is engineered for RT activity up to 65 °C, with an optimal reaction temperature of 55 °C. The reaction time is 10 minutes with a total protocol time of 13 minutes. This rapidly reduces time to result while working through challenging secondary RNA structures.
qScript Ultra SuperMix provides maximum yields across a wide variety of RNA input amounts, optimised for 2,5 μg to 1 pg total RNA input with even as little at 100 fg total RNA possible. This product demonstrates fine level discrimination and linear conversion of RNA to cDNA with a high copy reference gene at high input RNA amounts, which further emphasises accurate and critical expression levels. High efficiency and precision of RNA to cDNA conversion creates optimal cDNA yields for downstream use.
qScript Ultra SuperMix demonstrates low-copy sensitivity, linear dynamic range and higher yields of viral RNA in the presence of high levels of carrier RNA. This allows for low RNA input quantities to be detected with consistent yield and reduced dropout. The Quantabio PerfeCTa SYBR Green SuperMix and FastMix were both used for qPCR amplification.
qScript Ultra SuperMix can readily reverse transcribe RNA while overcoming common inhibitors found in blood, skin, plants, and RNA extraction carryover chemicals. The robust, engineered qScript Ultra reverse transcriptase allows for tolerance to many common reaction inhibitors. qScript Ultra SuperMix consistently produces high yields of cDNA even in the presence of these challenging inhibitors. The Quantabio PerfeCTa SYBR Green FastMix, low ROX was used for qPCR amplification.
qScript Ultra SuperMix can readily reverse transcribe long RNA transcripts in an unbiased manner, maintaining equivalent representation in the qPCR amplification curves throughout the length of the transcript. First strand cDNA synthesis using qScript Ultra SuperMix was performed with total RNA input levels of 100 ng, 10 ng, 1 ng and 100 pg. The Quantabio PerfeCTa SYBR Green FastMix was used for qPCR amplification.
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