MitoView™
Supplier: Biotium
MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.
- Cellular localization: Mitochondria
- For live or fixed cells: For live/intact cells
- Assay type/options: No-wash staining, Real-time imaging
- Cell permeability: Membrane permeant
- Abs/Em: 644/670 nm
- Detection channel: Cy®5, APC*
- Potential-dependent?: Partial (Dyes localize to the cytoplasm after mitochondrial depolarization, but still retain fluorescence.)
MitoView™ 650 is a far-red fluorescent mitochondrial dye that is not dependent on mitochondrial membrane potential. The dye has excitation/emission at 644/670 nm for the Cy®5 channel. Unlike MitoView™ 633, it does not bleed into the visible red channel, and so can be combined with red probes for multicolor imaging. The dye fluorescence is not lost after mitochondrial depolarization or fixation, but localization becomes non-specific.
Cy Dye is a registered trademark of GE Healthcare. MitoTracker is a registered trademark of Thermo Fisher Scientific.
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