Naphthol yellow S hydrate for microscopy, for the precipitation of amino acids and peptides, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
Warning

Synonyms: Flavianic acid disodium salt, Disodium 2,4-Dinitro-1-naphthol-7-sulphonate, 8-Hydroxy-5,7-dinitro-2-naphthalenesulphonic acid disodium salt, 2,4-Dinitro-1-naphthol-7-sulphonic acid disodium salt, Disodium 8-Hydroxy-5,7-dinitro-2-naphthalenesulphonate

M-Clarity™ quality level = MQ200

70540-100G 70540-25G
70540-100GEA 79.4 GBP
70540-100G 70540-25G
Naphthol yellow S hydrate for microscopy, for the precipitation of amino acids and peptides, Sigma-Aldrich®
Naphthol yellow S hydrate

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.


Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

Formula: C₁₀H₆N₂O₈S·xH₂O
MW: 358,19 g/mol (anhy)
Storage Temperature: Room Temperature
MDL Number: MFCD00003958
CAS Number: 846-70-8
EINECS: 212-690-2
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Specification Test Results

Appearance (colour) Light yellow to yellow and light orange to orange
Appearance (form) Powder
Thin layer chromatogr. Corresponds to standard
Solubility (colour) Yellow to very dark yellow and orange to very dark orange
Solubility (turbidity) --
Solubility (method) 0.5 g in 10 ml methanol/water 1:1
Wavelength (1) (UV) 423 - 433 nm
Extinction (1%/1 cm) (1) (UV) ≥275
Wavelength (2) (UV) 387 - 397 nm
Extinction (1%/1 cm) (2) (UV) ≥250
Solvent (UV) Water

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