Nigrosine, certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Acid Black 2

M-Clarity™ quality level = MQ200

198285-100G 198285-25G
198285-100GEA 97.9 GBP
198285-100G 198285-25G
Nigrosine, certified by the Biological Stain Commission, Sigma-Aldrich®
Nigrosine

Nigrosin,certified by the Biological Stain Commission (Acid black 2; Nigrosin water soluble).


Certified for use in Dorner′s spore stain. Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi. Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.

Storage Temperature: Room Temperature
MDL Number: MFCD00044681
CAS Number: 8005-03-6

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Specification Test Results

Appearance (Colour) Black
Appearance (Form) Powder or Crystals
Infrared spectrum Conforms to Structure
Wavelength Absorbance 290 - 300 nm
Wavelength 570 - 580 nm
Absorbance c = 0.02 g/L in 50% Ethanol
Biological Stain Commission, Commission Certified Number: Pass
Carbon 40 - 50% Typically
Nitrogen 7 - 9% Typically
TLC, Consistent With Control Conforms
Solubility (Turbidity), c = 0.1% in H2O Clear
Solubility (Colour) Dark Purple

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