Orange II ≥85 % (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Tropaeolin OOO-2, acid orange 7, acid orange A, D and C Orange No. 4, 4-(2-Hydroxy-1-naphthylazo)benzenesulphonic acid sodium salt, Orange II sodium salt, p-(2-Hydroxy-1-naphthylazo)benzenesulphonic acid sodium salt, Gold orange

M-Clarity™ quality level = MQ200

195235-100G 195235-25G
195235-100GEA 88.2 GBP
195235-100G 195235-25G
Orange II ≥85 % (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®
Orange II

Orange II sodium salt,(Certified by the Biological Stain Commission), Dye content ≥85 % (4-(2-Hydroxy-1-naphthylazo)benzenesulfonic acid sodium salt; Acid Orange 7; Acid Orange A; Orange II; Tropaeolin 000 No. 2).


Certified for use in the Wilson-Ezrin method for pituitary acidophils, as a counterstain following Heidenhain′s or Weigert′s hematoxylin on paraffin sections, and as a substitute for orange G in Floemming′s triple stain.

Formula: C₁₆H₁₁N₂NaO₄S
MW: 350,32 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00011657
CAS Number: 633-96-5
EINECS: 211-199-0

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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength, c = 0.01 g/L, H₂O 480 - 486 nm
Extinction Coefficient ≥26500
Wavelength 225 - 231 nm
Biological Stain Commission, Commission Certified Number: Pass
Dye Content ≥85%
Carbon 46.6 - 63.1%
Nitrogen 6.8 - 9.2%

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