Leuco crystal violet, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: 4,4',4''-Methanetriyltris(N,N-dimethylaniline), Tris(4-dimethylaminophenyl)methane, 4,4',4''-Methylidynetris(N,N-dimethylaniline)

M-Clarity™ quality level = MQ200

219215-25G 219215-5G
219215-25GEA 172 GBP
219215-25G 219215-5G
Leuco crystal violet, Sigma-Aldrich®
Leuco crystal violet

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.


Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colourless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet colour. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.


Caution: Photo-ionisation can cause color variations.

Formula: C₂₅H₃₁N₃
MW: 373,54 g/mol
Melting Pt: 175,..177 °C (lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00008314
CAS Number: 603-48-5
EINECS: 210-043-9

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Specification Test Results

Appearance (Colour); White to Grey to Lavender Conforms to Requirements
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength, C = 0.01 G/L IN 0.1N HCL IN MEOH 257 - 263 nm
Extinction Coefficient ≥38000
Wavelength 587 - 593 nm
Absorbance ≤0.01
Carbon 77.6 - 83.2%
Nitrogen 10.8 - 11.6%

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