Acid red 52, Technical Grade, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Food Red No.106, Sodium 4-(3,6-bis(diethylamino)xanthylium-9-yl)benzene-1,3-disulphonate, Kiton Red S, Sulphorhodamine B sodium salt, Lissamine rhodamine B-200

M-Clarity™ quality level = MQ200

S9012-25G S9012-5G
S9012-25GEA 413 GBP
S9012-25G S9012-5G
Acid red 52, Technical Grade, Sigma-Aldrich®
Acid red 52

Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.


Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening. It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts. It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.

Formula: C₂₇H₃₀N₂O₇S₂
MW: 580,65 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00010180
CAS Number: 3520-42-1
EINECS: 222-529-8
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Specification Test Results

Appearance (Form) Powder or Crystals
Infrared spectrum Conforms to Structure
Wavelength, In Methanolic Sodium Hydroxide 554 - 560 nm
Extinction Coefficient ≥85000
Carbon ≥40.8 %
Nitrogen ≥3.5 %
Dye Content ≥72 %
Recommended Retest Period 5 Years

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