bisBenzimide H 33258 trihydrochloride (Hoechst 33258) ≥98% (by HPLC, TLC), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
Warning

Synonyms: 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride, BBIH, HOE 33258, Bisbenzimide, 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole trihydrochloride, Hoechst 33258

M-Clarity™ quality level = MQ200

B2883-100MG B2883-1G B2883-25MG B2883-500MG
B2883-100MGEA 126 GBP
B2883-100MG B2883-1G B2883-25MG B2883-500MG
bisBenzimide H 33258 trihydrochloride (Hoechst 33258) ≥98% (by HPLC, TLC), Sigma-Aldrich®
bisBenzimide H 33258 trihydrochloride (Hoechst 33258)

bisBenzimide H 33258 has been used for nuclear staining in cells. bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.

Formula: C₂₅H₂₄N₆O·3HCl
MW: 533,88 g/mol
Melting Pt: 280 °C
Storage Temperature: Freezer
MDL Number: MFCD00012679
CAS Number: 23491-45-4
EINECS: 245-690-6
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Specification Test Results

Appearance (Form) Powder
Appearance (Colour) Yellow to Dark Yellow
Solubility (Solvent) Water
Solubility (Conc) 9.80 - 10.20 mg/ml
Solubility (Turbidity) Clear
Solubility (Colour) Yellow to Brown
NMR (Solvent) D2O
Identity by NMR Consistent
NMR (Residual Solvent 1) Methanol
NMR (% Solvent 1) ≤1.00%
NMR (Residual Solvent 2) E/E
NMR (% Solvent 2) ≤1.00%
NMR (Total % Resid.Solvents) ≤1.00%
Purity (TLC) ≥98.00%
Purity (HPLC) ≥98.00%

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