Alcian blue solution, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
M-Clarity™ quality level = MQ100
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans.[5] It is also used for the staining of bone and cartilage.
Formula:
C₅₆H₆₈Cl₄CuN₁₆S₄ MW: 1298,86 g/mol Storage Temperature: Room Temperature |
MDL Number:
MFCD00010720 CAS Number: 33864-99-2 |
Specification Test Results
Appearance (Colour) | Dark Blue to Very Dark Blue |
Appearance (Form) Sediment free |
Liquid |
Wavelength | |
Absorbance 1:400 in H2O |
0.45 - 0.60 Abs UN |
pH | 2.4 - 2.6 |
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