Aniline blue solution 2,5% in 2% acetic acid for dye, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: China Blue, CN DcK-11, CI 42755, Marine Blue V, Water Blue, Blackley Blue, Disodium hydrogen aminomethyl[[4-[(sulphonatophenyl)amino]phenyl][4-[(sulphonatophenyl)imino]cyclohexa-2,5-dien-1-ylidene]methyl]benzenesulphonate, Cotton Blue, Acid Blue 22

M-Clarity™ quality level = MQ200

B8563-250ML B8563-500ML
B8563-250MLEA 57 GBP
B8563-250ML B8563-500ML
Aniline blue solution 2,5% in 2% acetic acid for dye, Sigma-Aldrich®
Aniline blue

Aniline blue is used to stain collagen fibers in tissue sections using the Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. It has also been used to stain and visualize glycosylated proteins.


Aniline blue (also known as cotton blue and ink blue) is a component of commonly used polychrome stains. It is used to stain collagen fibers in tissue sections using Masson's trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Heidenhain's AZAN stain and the Mallory's polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori's one-step trichrome stain and Mallory's connective tissue stain for tissue including kidney and intestine.

Formula: C₃₂H₂₅N₃Na₂O₉S₃
MW: 737,74 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00036130
CAS Number: 28631-66-5

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Specification Test Results

Appearance Dark Blue, Sediment Free
UV/VIS Absorbance 0.21 - 0.37
Lambda max 597-603 nm

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