EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], powder, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Bis(2,5-dioxopyrrolidin-1-yl) O,O'-ethane-1,2-diyl disuccinate, EGS, Ethylene glycol disuccinate di(N-succinimidyl) ester, Ethylene glycol bis(succinimidyl succinate), Di(N-succinimidyl) ethylene glycol disuccinate

M-Clarity™ quality level = MQ200

E3257-1G E3257-250MG
E3257-1GEA 523 GBP
E3257-1G E3257-250MG
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], powder, Sigma-Aldrich®
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)]

Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) has been used as a cross-linking agent.


  • The ethylene glycol linkage is cleavable by hydroxylamine


Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is a homobifunctional, cleavable cross-linking reagent. It is mainly coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer.

Formula: C₁₈H₂₀N₂O₁₂
MW: 456,36 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00049057
CAS Number: 70539-42-3
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Specification Test Results

Appearance (Colour) White to Off-White
Appearance (Form) Powder
Solubility (Colour) Colorless to Faint Yellow
Solubility (Turbidity)
50 mg/mL in HOAc:H2O (1:1)
Clear to Hazy
Proton NMR spectra Conforms to Structure
13C NMR SPECTRA Conforms to Structure
Carbon 46.1 - 48.6 %
Nitrogen 5.8 - 6.6 %

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