Stains-all ~95%, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
Synonyms:
1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
M-Clarity™ quality level = MQ200
Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.
Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca2+ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).
As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.
Caution: Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.
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Formula:
C₃₀H₂₇BrN₂S₂ MW: 559,584 g/mol Storage Temperature: Refrigerator |
MDL Number:
MFCD00078788 CAS Number: 7423-31-6 |
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Specification Test Results
| Appearance (Colour) | Report Result |
| Appearance (Form) | Report Result |
| Solubility (Colour) | 40Mg Plus 4.0Ml Of Chloroform |
| Solubility (Method) | 40Mg Plus 4.0Ml Of Chloroform |
| Carbon Content | 60.5 - 65.7 % |
| Nitrogen Content | 4.5 - 5.5 % |
| Infrared Spectrum | Conforms To Structure |
| Dye Content | ~ 95 % |
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