NucSpot® Far-Red 1000X in DMSO
Supplier: Biotium
NucSpot® Far-Red was designed to be an improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.
- Designed as an improvement replacement for 7-AAD
- Less bleed into the PE-Texas Red® channel compared to 7-AAD
- Selective detection of dead cells by flow cytometry in the PE-Cy®5 or APC channel
- Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells
- Substitute for 7-AAD in any standard protocol
- λEx/λEm (with DNA) = 597/667 nm
NucSpot® Far-Red is an improved alternative to the popular flow cytometry dead cell dye 7-AAD. It has red-shifted fluorescence emission compared to 7-AAD, for less bleed-through fluorescence in the PE-Texas Red® channel.
Like 7-AAD, NucSpot® Far-Red can be excited with the 488 nm laser for detection in the PE-Cy®5 channel, or excited with the 633 nm laser for detection in the APC channel. NucSpot® Far-Red has similar brightness to 7-AAD in the PE-Cy®5 channel, and is much brighter in the APC channel.
NucSpot® Far-Red is excluded by live cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. Like 7-AAD, NucSpot® Far-Red also can be used for DNA content analysis of cell cycle by flow cytometry in fixed and permeabilized cells without the need for RNase-treatment, unlike propidium iodide.
Cy dye is a registered trademark of GE Healthcare; Texas Red is a registered trademark of Thermo Fisher Scientific.
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