NucSpot® Far-Red 1000X in DMSO

Supplier: Biotium

40085-T 40085
40085-TEA 121 GBP
40085-T 40085.
NucSpot® Far-Red 1000X in DMSO
NucSpot®

NucSpot® Far-Red was designed to be an improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.


  • Designed as an improvement replacement for 7-AAD
  • Less bleed into the PE-Texas Red® channel compared to 7-AAD
  • Selective detection of dead cells by flow cytometry in the PE-Cy®5 or APC channel
  • Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells
  • Substitute for 7-AAD in any standard protocol
  • λEx/λEm (with DNA) = 597/667 nm


NucSpot® Far-Red is an improved alternative to the popular flow cytometry dead cell dye 7-AAD. It has red-shifted fluorescence emission compared to 7-AAD, for less bleed-through fluorescence in the PE-Texas Red® channel.


Like 7-AAD, NucSpot® Far-Red can be excited with the 488 nm laser for detection in the PE-Cy®5 channel, or excited with the 633 nm laser for detection in the APC channel. NucSpot® Far-Red has similar brightness to 7-AAD in the PE-Cy®5 channel, and is much brighter in the APC channel.


NucSpot® Far-Red is excluded by live cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. Like 7-AAD, NucSpot® Far-Red also can be used for DNA content analysis of cell cycle by flow cytometry in fixed and permeabilized cells without the need for RNase-treatment, unlike propidium iodide.


Cy dye is a registered trademark of GE Healthcare; Texas Red is a registered trademark of Thermo Fisher Scientific.

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