Haematoxylin modified solution Gill III used in nuclear staining, Sigma-Aldrich®

Supplier: Merck
Danger

Synonyms: HydroxybrasilinDcH-48Natural Black I

1.05174.2500EA 83.5 GBP
1.05174.2500 1.05174.0500 1.05174.1000
Haematoxylin modified solution Gill III used in nuclear staining, Sigma-Aldrich®
Haematoxylin staining solutions

Haematoxylin solution modified acc. to Gill III


In haematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready to use staining solutions, such as Mayer’s hemalum solution or haematoxylin solution modified according to Gill III. These solutions do not need to be filtrated prior use and the dye is oxidised in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e, for proteins, collagen, keratin and intercellular substances, can be performed with alcoholic or aqueous Eosin Y solution 0,5%. A 500 ml bottle is sufficient for between 1500 to 2500 slides. It is registered as an IVD and CE product.

Formula: C₁₆H₁₄O₆
MW: 302,28 g/mol
Density: 1,07 g/cm3 (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00078111
CAS Number: 517-28-2
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Specification Test Results

Suitability for microscopy passes test
Nuclei dark blue to dark violet
Cytoplasm pink to red
intercellular substances pink to red
Erythrocytes yellow to orange
Eosinophilic cytoplasm pink

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