MitoView™ 663 mitochondria dye, for fluorescence microscopy
Supplier: Biotium
MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.
- Cellular localization: Mitochondria
- For live or fixed cells: For live/intact cells
- Assay type/options: No-wash staining, Real-time imaging
- Cell permeability: Membrane permeant
- Abs/Em: 622/648 nm*
- Detection channel: Cy®5, APC*
- Potential-dependent?: Yes
MitoView™ 633 is a far-red fluorescent mitochondrial dye with absorbance/emission at 622/648 nm. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. The dye is designed for use in live cells, and is not fixable. Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.
* MitoView™ 633 also has red fluorescence in the Cy®3 channel and is not recommended for use with other red dyes.
Cy Dye is a registered trademark of GE Healthcare. MitoTracker is a registered trademark of Thermo Fisher Scientific.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...