MitoView™ 405 mitochondria dye, for fluorescence microscopy
Supplier: Biotium
MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.
- Cellular localization: Mitochondria
- For live or fixed cells: For live/intact cells
- Assay type/options: No-wash staining, Real-time imaging
- Cell permeability: Membrane permeant
- Abs/Em: 398/440 nm
- Detection channel: DAPI
- Potential-dependent?: Partial (Dyes localize to the cytoplasm after mitochondrial depolarization, but still retain fluorescence.)
MitoView™ 405 is a blue fluorescent mitochondrial dye with absorbance/emission at 398/440 nm, suitable for detection by confocal microscopy or flow cytometry using settings for DAPI or Pacific Blue®. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Mitochondrial localization is dependent on mitochondrial membrane potential; when membrane potential is disrupted the dye relocalizes to the cytoplasm, but still retains fluorescence. The dye is designed for use in live cells and is not fixable. MitoView™ 405 is a replacement for our original MitoView™ Blue, and has improved photostability.
Note: MitoView™ Blue (70052) has been discontinued and replaced by the improved MitoView™ 405. The original MitoView™ Blue will be available for purchase as a special order while supplies last. Contact techsupport@biotium.com to inquire about availability.
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