Rhod-2 AM (Bromide) fluorescent indicator, for calcium determination
Supplier: Biotium
Synonyms:
Rhod-2 AM ester, 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N',N'-tetraacetic acid, tetraacetoxy methyl ester . Br
Membrane-permeant form of Rhod-2 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 is often used as a dispersing agent to facilitate dye loading. Rhod-2-AM itself does not bind Ca2+ , but it is readily hydrolyzed to Rhod-2 by endogenous esterases once it enters cells.
- Absorption/Emission 556nm/576nm (low or high [Ca2+] )
- Extinction Coefficient 80,000 M-1 cm-1 (556 nm)
- Cell permeability: Membrane permeant
- Red solid soluble in DMSO
- Store at -20°C and protect from light, especially in solution
Formula:
C₅₂H₅₉BrN₄O₁₉ MW: 1124,0 g/mol Storage Temperature: Freezer |
CAS Number:
145037-81-6 |
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