Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9% (dried basis), Ultrapure Bioreagent for molecular biology, J.T.Baker®

Supplier: Avantor
Warning

Synonyms: 2-Amino-2-(hydroxymethyl)propane-1,3-diol(tris), Tromethamine, Tris(hydroxymethyl)methylamine, 2-Amino-2-(hydroxymethyl)propane-1,3-diol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trizma(R) base, TRIS base, THAM

4109-01EA 115 GBP
4109-01 4109-02 4109-06 4109-07 4109-09 4109-F7
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9% (dried basis), Ultrapure Bioreagent for molecular biology, J.T.Baker®
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Buffer for molecular biology and liquid chromatography.


  • Lot analysis on label

Formula: NH₂C(CH₂OH)₃
MW: 121,14 g/mol
Boiling Pt: 357 °C (1013 hPa)
Melting Pt: 169…172 °C
Density: 1,328 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00004679
CAS Number: 77-86-1
EINECS: 214-684-5
Merck Index: 13,09842
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Specification Test Results

For Laboratory,Research,or Manufacturing Use ≥99.9%
Assay (C₄H₁₁NO₃) (dried basis) ≥99.9 %
Assay (C₄H₁₁NO₃) 99.0 - 101.0 %
Appearance (White crystals) Passes Test
Absorbance of a 1M Solution - 260 nm ≤0.06
Absorbance of a 1M Solution - 280 nm ≤0.06
Absorbance of a 1M Solution - 400 nm ≤0.01
RNase Activity (None Detected) Passes Test
DNase Activity (None Detected) Passes Test
Protease Activity (None Detected) Passes Test
pH of 0.1M Solution at 25°C 10.0 - 11.0
Insoluble Matter ≤0.005 %
Water (H₂O)(by Karl Fischer titrn) ≤0.3 %
Trace Impurities - Arsenic (As) ≤1 ppm
Trace Impurities - Calcium (Ca) ≤1 ppm
Trace Impurities - Copper (Cu) ≤1 ppm
Trace Impurities - Iron (Fe) ≤1 ppm
Trace Impurities - Lead (Pb) ≤1 ppm
Trace Impurities - Magnesium (Mg) ≤1.000 ppm
Trace Impurities - Manganese (Mn) ≤1 ppm
Identification A Passes Test
Identification B Passes Test
Identification C Passes Test
Description Passes Test
pH (1 in 20) 10.0 - 11.5
Loss on Drying ≤1.0 %
Residue after Ignition ≤0.10 %
Heavy Metals (as Pb) ≤0.001 %
Melting Range(Lower) 168 - 172 °C
Melting Range(Upper) 168 - 172 °C
No Class 1,2,3 or other solvents are used or produced in the
manufacturing or purification of the product.
Material also meets the chemical specifications of Tromethamine, U.S.P.
Manufactured exclusively for Avantor Performance Materials, LLC., by BioSpectra, Inc., Stroudsburg, PA

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