D-(+)-Biotin ≥97.5%, white crystalline powder cell culture reagent

Supplier: MP Biomedicals

Synonyms: Vitamin H, D-Biotin, Vitamin B8, 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, Vitamin B7

0219463405 0219463401 02194634.5
ICNA0219463405EA 580 GBP
ICNA0219463405 ICNA0219463401 ICNA02194634.5
D-(+)-Biotin ≥97.5%, white crystalline powder cell culture reagent
D(+)-Biotin

Storage: +4 °C
D-Biotin is a growth factor present in small amounts in every living cell. It is involved in naturally occurring carboxylation reactions. It occurs mainly bound to proteins or polypeptides. It is more abundant in the liver, kidney, pancreas, yeast and milk. Biotin levels are higher in cancerous tumors than in normal tissues. It is inactivated by binding to avidin.
D-Biotin may be used to elute proteins from avidin/streptavidin resins. It is widely used for dietary supplements and fortified foods. It is also used for tablets and hard-shell capsule preparation due to its pharmaceutical properties.
Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.
Soluble in water (22 mg/100 mL), ethanol (80 mg/100 mL), more soluble in hot water and in dilute alkalies; insoluble in other common organic solvents. Soluble in 2 M Ammonium hydroxide (50 mg/mL - clear, colorless solution), dimethylformamide (1.7 mg/mL).


  • Cell Culture Reagent Grade
  • Refractive Index: 90.5 ° (C=2, 0.1mol/L NaOH) (Lit.)
  • Presentation: White Crystalline Powder
  • Isoelectric Point (pl): 3.5 (lit.)
  • Reaction (0.02% aq soln)- Very Slightly Acidic to Litmus

Formula: C₁₀H₁₆N₂O₃S
MW: 244,31 g/mol
Boiling Pt: 573,6 °C (760 mmHg)
Melting Pt: 223…233 °C
Density: 1,267 g/cm³ (lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00005541
CAS Number: 58-85-5
EINECS: 200-399-3
Merck Index: 13,01231

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Specification Test Results

Identity Test Passes
Purity ≥97.5%
FTIR Conforms to Standard
Solubility (0.02% aq soln) Clear Solution
Reaction (0.02% aq soln) Very Slightly Acid to Litmus
Optical Rotation (c=2, 0.1 N NaOH) +89 to +93°
Organic Volatile Impurities Passes

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