Sclerostin-LRP interaction screening system, LEADING LIGHT®
Supplier: ENZO LIFE SCIENCES AG
Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens.
- High sensitivity chemiluminescent readout (signal-to-noise ratio of ≥10)
- Rapid protocol yields results in just 2,5 hours
- No cell lines or transfection reagents required
- Reproducible, high-throughput amenable assay (Z’-factor 0.871)
- Amenable for high-throughput screening applications
The LEADING LIGHT® Sclerostin Screening System is a 1 x 96 well plate assay based on Sclerostin–LRP interaction. This system contains engineered LRP5-alkaline phosphatase (ALP) and human Sclerostin immobilized in a multi-well plate. LRP5-alkaline phosphatase binds to Sclerostin coated on the bottom of the well and is detected by the activity of alkaline phosphatase remaining after a washing step. This system can be used for drug screening to identify small molecule compounds, antibodies, DNA aptamers and peptides capable of modulating sclerostin-LRP interaction.
Ordering information: Contains Sclerostin microtiter plate, LRP5-ALP fusion protein concentrate, ALP concentrate (negative control), PBS solution, Blocking agent, wash buffer, acid green 25, ALP substrate reagent, plate sealer.
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