β-Nicotinamide adenine dinucleotid, oxidised form (NAD, oxidised form) ≥98%, white powder cell culture reagent
Supplier: MP Biomedicals
Synonyms:
Nadide, NAD+, Nicotinamide adenine dinucleotide, oxidized
Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.
- Cell Culture Reagent Grade
- Extinction Coefficient (E1%): EmM = 18.0 (260 nm, pH 7) (Lit.)
- Presentation: White Powder
- Soluble in water (1%).
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Formula:
C₂₁H₂₇N₇O₁₄P₂ MW: 663,43 g/mol Melting Pt: 160 °C (lit.) Storage Temperature: Freezer |
MDL Number:
MFCD00036253 CAS Number: 53-84-9 |
Specification Test Results
| Identity Test | Passes |
| Purity | ≥98% |
| FTIR | Conforms to Standard |
| pH (1% aq soln) | 2-4 |
| I max (water) | 259 ± 3 nm |
| OD250/OD260 | 0.8 ± 0.15 |
| OD280/OD260 | 0.2 ± 0.15 |
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