Bovine hyaluronidase (from Testes)

Supplier: MP Biomedicals

0210074080 0210074090 0210074091
ICNA0210074080EA 125 GBP
ICNA0210074080 ICNA0210074090 ICNA0210074091
Bovine hyaluronidase (from Testes)
Enzymes

Hyaluronidase is a glycoprotein containing 5% mannose and 2,17% glucosamine, it catalyses the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a molecular mass of 14 kDa each.


  • Gene ID: 281838
  • Gene symbol and aliases: HYAL1, HYAL2
  • Uniprot number: Q8SQG8
  • Enzyme Commission Number E.C. 3.2.1.35
  • Inhibitors: Fe²⁺, Fe³⁺, Mn²⁺ and Cu²⁺
  • Sodium chloride acts as a stabiliser
  • Dissolve 10 mg hyaluronic acid in 25 ml 0,1 M sodium phosphate buffer: pH 5,3 with 0,15 M sodium chloride


It degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.


Testicular hyaluronidase hydrolyses the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated.


It is often used in conjunction with collagenase to dissociate the extracellular matrix between cells of animal tissue, in order to release viable cells for use in tissue culture. It may also be used to clarify synovial fluids in order to make cell counts possible.


Unit definition: Amount of enzyme which liberates one micromole of N-acetylglucosamine per minute at 37 °C and pH 4,0.

Order Now


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR