Propidium iodide, dark red powder

Supplier: MP Biomedicals
Danger

Synonyms: PI3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide3,8-Diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide

ICNA0219545880EA 239 GBP
ICNA0219545880 ICNA0219545883 ICNA0219545810 ICNA0219545825
Propidium iodide, dark red powder
Propidium iodide

Propidium iodide is a fuorescent stain for nucleic acids.


Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.


Propidium iodide (PI) binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4 to 5 base pairs of DNA. PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining. Once the dye is bound to nucleic acids, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30 to 40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue. Although its molar absorptivity (extinction coefficient) is relatively low, PI exhibits a sufficiently large Stokes shift to allow simultaneous detection of nuclear DNA and fluorescein-labeled antibodies, provided the proper optical filters are used. Propidium iodide is suitable for fluorescence microscopy, confocal laser scanning microscopy, flow cytometry and fluorometry.


Soluble at 1 mg/ml in water.

Formula: C₂₇H₃₄I₂N₄
MW: 668,4 g/mol
MDL Number: MFCD00011921
CAS Number: 25535-16-4

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Specification Test Results

Identity Test Passes
FTIR Conforms to Standard
UV/VIS: l max (water) 493 ± 4 nm

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