Nitrocefin ≥95%

Supplier: Cayman Chemical

Synonyms: (6R,7R)-7-[[(E)-4-(2,4-Dinitrophenyl)-2-(2-thienyl)but-3-enoyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 3-(2,4-DINITROSTYRYL)-(6R, 7R)-7-(2-THIENYLACETAMIDO)-CEPH-3-EM-4 CARBOXYLIC ACID, E-ISOMER, N-[3-[2-(2,4-DINITRO-PHENYL)-VINYL]-8-OXO-5-THIA-1-AZA-BICYCLO[4.2.0]OCT-7-YL]-2-THIOPHEN-2-YL-ACETAMIDE

15424-10 15424-5 15424-1 15424-25
CAYM15424-10EA 424 GBP
CAYM15424-10 CAYM15424-5 CAYM15424-1 CAYM15424-25
Nitrocefin ≥95%
Nitrocefin

Nitrocefin is a chromogenic cephalosporin substrate commonly used to detect β-lactamases in bacteria. The presence of β-lactamase activity is indicated by the appearance of a red color that is proportional in intensity to the original concentration of nitrocefin.


The generation of β-lactamases by bacteria affords resistance to several classes of β-lactam antibiotics, including penicillins and cephalosporins.

Formula: C₂₁H₁₆N₄O₈S₂
MW: 516,499 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00864896
CAS Number: 41906-86-9

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Specification Test Results

Purity ≥95%
Formal Name (6R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7R-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
Synonyms Nitrocephin
Formulation A Crystalline Solid
λmax 231, 286, 379 nm
SMILES O=C(O)C1=C(CS[C@@]([C@@H]2NC(CC3=CC=CS3)=O)([H])N1C2=O)/C=C/C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O
InChI Code 1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1
InChI Key LHNIIDJCEODSHA-OQRUQETBSA-N
Storage −20 °C
Stability 2 years from the QC date provided on the Certificate of Analysis, when stored properly

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