Sulpho-NHS (N-Hydroxysulphosuccinimide sodium salt), Pierce™

Supplier: Thermo Fisher Scientific

Synonyms: N-Hydroxy sulphosuccinimide sodium salt, Hydroxy-2,5-dioxopyrrolidine-3-sulphonic acid sodium salt, N-Hydroxysulphosuccinimide sodium salt, Sulfo NHS, Sulpho-N-Hydroxysuccinimide

24510 24525
PIER24510EA 418 GBP
PIER24510 PIER24525
Sulpho-NHS (N-Hydroxysulphosuccinimide sodium salt), Pierce™
Sulpho-NHS (N-Hydroxysulphosuccinimide sodium salt)

Pierce™ Sulfo-NHS (N-hydroxysulfosuccinimide) is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilisation methods.


  • Improve efficiency of EDC coupling reactions
  • Water-soluble and membrane-impermable
  • Activate nanoparticles with amine-reactive Sulpho-NHS esters
  • Crosslink proteins to carboxyl-coated beads or surfaces more efficiently


Derivatives of Sulpho-NHS are easily synthesised by mixing the Sulpho-NHS with a carboxyl-containing molecule and a dehydrating agent. The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₄H₄NNaO₆S
MW: 217,13 g/mol
MDL Number: MFCD00043100
CAS Number: 106627-54-7

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Specification Test Results

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Carbodiimide
Spacer Short
Form Powder
Quantity 500 mg
Type Crosslinker
Solubility Water

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