3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate
Supplier: Rockland Immunochemicals
Synonyms:
TMB
, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine
TMB ELISA peroxidase substrate (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow.
- This substrate will produce a soluble blue end product read at 370 nm or 655 nm. It's incubation time varies depending on the assay conditions.
TMB ELISA peroxidase substrate (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate used for antibody reactivity in ELISA experiments.
TMB ELISA peroxidase substrate is designed for use in immunology and cancer research.
Delivery information: Supplied as a ready to use clear to very light blue colour liquid.
Formula:
C₁₆H₂₀N₂ MW: 240,35 g/mol |
MDL Number:
MFCD00007748 CAS Number: 54827-17-7 |
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