Calcein AM

Supplier: Corning

Synonyms: Calcein-AM, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester, Calcein acetoxymethyl ester, Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylene))bis(azanetriyl))tetraacetate

354216 354217
734-1434EA 291 GBP
734-1434 734-1435
Calcein AM
Calcein AM

The fluorescent dye calcein acetoxymethylester (calcein AM) can be used to label cells when performing analyses such as tumour cell invasion, endothelial cell migration, endothelial cell tubulogenesis, and other cell-based assays.


  • Non fluorescent cell viability indicator
  • Cell permeant compound that is hydrolysed by intracellular esterases in the fluorescent anion calcein


Calcein AM can be used to fluorescently pre- and post-label viable cells and perform kinetic and endpoint experiments, respectively.


The fluorescent dyes, calcein acetoxymethylester (calcein AM), can be used with Falcon* Corning FluoroBlok* cell culture inserts and insert systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, and other cell-based assays


Caution: NOTE: Cells are known to tolerate different fluorescent dyes for varying amounts of time in culture. Cells labelled with calcein AM should be exposed to the dye for less than eight hours.

Formula: C₄₆H₄₆N₂O₂₃
MW: 994,86 g/mol
MDL Number: MFCD05861516
CAS Number: 148504-34-1

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