dsDNA quantitation kits, AccuBlue® and AccuClear®

Supplier: Biotium

31006 31008 31007 31008-T 31009 31009-T 31027 31027-T 31028 31029 31006-T 31007C 31007-T 31028-T 31006C 31029C
31006.EA 404 GBP
31006. 31008. 31007. 31008-T 31009. 31009-T 31027. 31027-T 31028. 31029. 31006-T 31007C 31007-T 31028-T 31006C 31029C
dsDNA quantitation kits, AccuBlue® and AccuClear®
Assays Nucleic Acid Assays

AccuBlue® and AccuClear® dsDNA Quantitation Kits offer sensitive and selective detection of double-stranded DNA in a 96-well microplate format.


  • Allow accurate quantitation of purified double-stranded DNA (dsDNA) samples across a wide range of concentrations
  • Compatible with plate readers and handheld fluorometers


Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for dsDNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.


AccuBlue® broad range assay: Broad linear range with blue fluorescence
The AccuBlue® broad range assay has a wide dynamic range of 2 to 2000 ng per well of a 96 well plate (0,2 to 200 ng/µl of sample). The standard curve can be extended to 4000 ng/well with slight compromise in linearity. Excitation/emission wavelengths: 350/460 nm (blue fluorescence).


AccuBlue® high sensitivity assay: Non-toxic and non-mutagenetic DNA quantitation dye
The AccuBlue® high sensitivity assay has a linear range of 0,2 to 100 ng dsDNA per well of a 96 well plate (0,02 to 10 ng/µl of sample). Excitation/emission wavelengths: 500/530 nm (green fluorescence).


AccuClear® ultra high sensitivity assay: Versatile kit with high sensitivity and wide linear range
The AccuClear® ultra high sensitivity assay has a linear range of 30 pg to 250 ng dsDNA per well of a 96-well plate. Excitation/emission wavelengths: 460/507 nm (green fluorescence).


Note: AccuBlue® NextGen assay for quantitation of ultra-low amounts of DNA in precious or dilute samples is also available (see Cat. No. 31060). The AccuBlue® NextGen assay has a linear range of 1 pg to 3 ng (dependent on instrument sensitivity) dsDNA per well of a 96-well plate (0,5 pg/µl to 300 pg/µl of sample). Excitation /emission wavelengths: 468/507 nm (green fluorescence).


Delivery information: AccuBlue®and AccuClear® kits include quantitation solution and calf thymus dsDNA standards. Stand-alone AccuBlue® and AccuClear® quantitation solutions are available for users who prefer to use their own DNA standards.

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