Osmium tetraoxide 4% in aqueous solution, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Danger
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Synonyms:
Osmium (VIII) oxide, Osmic acid anhydride, Osmic acid
M-Clarity™ quality level = MQ100
Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.
Osmium tetroxide solution has been used as a fixative in scanning and transmission electron microscopy.
Formula:
O₄Os MW: 254,23 g/mol Density: 1,0 g/ml at 20 °C Storage Temperature: Refrigerator |
MDL Number:
MFCD00011150 CAS Number: 20816-12-0 EINECS: 244-058-7 UN: 3287 ADR: 6.1,II |
Specification Test Results
Assay | 4.0% OsO₄ in water |
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