Fluorescein diacetate cell viability stain, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Di-O-acetylfluorescein, 3,6-Diacetoxyfluoran, 3-Oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate

M-Clarity™ quality level = MQ200

F7378-100G F7378-10G F7378-25G F7378-5G
F7378-100GEA 606 GBP
F7378-100G F7378-10G F7378-25G F7378-5G
Fluorescein diacetate cell viability stain, Sigma-Aldrich®
Fluorescein diacetate

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Formula: C₂₄H₁₆O₇
MW: 416,38 g/mol
Melting Pt: 200,..203 °C (lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00005062
CAS Number: 596-09-8
EINECS: 209-877-6
https://digitalassets.avantorsciences.com/adaptivemedia/rendition?id=7755e80a40ba77a5c7ea98959a1302a6b8024d53&vid=7755e80a40ba77a5c7ea98959a1302a6b8024d53&prid=web&clid=SAPDAM

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Specification Test Results

Appearance (Colour) White to Faint Yellow
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Solubility (Turbidity), c = 25 mg/ml; Acetone Clear
Solubility (Colour) Colourless to Faint Yellow
Emission Wavelength, In 0.1 N Sodium Hydroxide 488.0 - 494.0 nm
EMM ≥86.000
Carbon 68.5 - 69.9 %

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