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Product Image-M1662

Maxima™ H minus cDNA synthesis master mix

Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.
Catalog Number: M1662
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific

Certificates


Product Image-M1682

Maxima™ H minus cDNA synthesis master mix, with dsDNase

Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.
Catalog Number: M1682
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-K1642

Maxima™ First strand cDNA synthesis kit

Description: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.
Catalog Number: K1642
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-27-9266-01

RT-PCR beads, illustra™ Ready-To-Go™

Description: Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.
Catalog Number: 27-9266-01
UOM: 1 * 100 Tests
Supplier: Cytiva
Image Unavailable-71978-3

One step RT-PCR master mix kit

Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
Catalog Number: 71978-3
UOM: 1 * 1 KIT
Supplier: Merck Millipore (Novagen)
Product Image-EP0741

Reverse transcriptase, Maxima™

Description: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.
Catalog Number: EP0741
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-27-9261-01

First-strand cDNA Synthesis kit

Description: Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for <i>in vitro</i> amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.
Catalog Number: 27-9261-01
UOM: 1 * 55 Tests
Supplier: Cytiva
Product Image-K1622

RevertAid™ First strand cDNA synthesis kit

Description: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.
Catalog Number: K1622
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-733-1098

RT-PCR kits, Verso™

Description: The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.
Catalog Number: 733-1098
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-K1652

Maxima™ H minus first strand cDNA synthesis kits

Description: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.
Catalog Number: K1652
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-EP0452

RevertAid™ H Minus Reverse Transcriptase

Description: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.
Catalog Number: EP0452
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Image Unavailable-ICNA11NTPMX105

dNTP mix, MP Biomedicals

Description: This dNTP mix with dATP, dCTP, dGTP, dTTP is suitable for use in PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, DNA sequencing and labeling.
Catalog Number: ICNA11NTPMX105
UOM: 1 * 10 µmol
Supplier: MP Biomedicals
Product Image-K1632

RevertAid™ H minus first strand cDNA synthesis kits

Description: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.
Catalog Number: K1632
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-27-9264-01

PCR beads, Ready-To-Go™ You-Prime First-Strand Beads

Description: Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.
Catalog Number: 27-9264-01
UOM: 1 * 50 Tests
Supplier: Cytiva

MSDS


Image Unavailable-K1612

First strand cDNA synthesis kit

Description: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
Catalog Number: K1612
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Image Unavailable-K0231

Maxima™ Probe qPCR Master Mix (2X)

Description: The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
Catalog Number: K0231
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
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