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You Searched For: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Human Untagged FBXL17 Clone

Catalog Number: (ORIGSC105850)
Supplier: OriGene
Description: FBXL17 (untagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17). The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).
UOM: 1 * 10 µG

New Product


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Human Tagged ORF Clone, SPCA2 (ATP2C2)

Catalog Number: (ORIGRG239585)
Supplier: OriGene
Description: ATP2C2 (tGFP-tagged) - Human ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2), transcript variant 3.
UOM: 1 * 10 µG
Product Image-OZBIPL00050

Transfection control plasmids, pVectOZ

Supplier: OZ BIOSCIENCES
Description: pVectOZ transfection plasmids are engineered in an optimised backbone and are ideal for all transfections.

Product Image-PIER88871

Cell-free protein expression vectors, T7, mammalian

Supplier: Thermo Fisher Scientific
Description: The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the<i> in vitro</i> translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for<i> in vitro</i> transcription and translation.

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pET-14b DNA, Novagen®

Catalog Number: (69660-3)
Supplier: Merck Millipore (Novagen)
Description: The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.
UOM: 1 * 10 µG
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Vector, pET-24(+) DNA

Catalog Number: (69772-3)
Supplier: Merck Millipore (Novagen)
Description: pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available
UOM: 1 * 10 µG
Image Unavailable-69752-3

Vector, pET-24d(+) DNA

Catalog Number: (69752-3)
Supplier: Merck Millipore (Novagen)
Description: The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.
UOM: 1 * 10 µG

MSDS


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pET-30a(+) DNA, Novagen®

Catalog Number: (69909-3)
Supplier: Merck Millipore (Novagen)
Description: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.
UOM: 1 * 10 µG
Image Unavailable-ORIGMG225346

Slc7a11 (NM_011990) mouse tagged ORF clone

Catalog Number: (ORIGMG225346)
Supplier: OriGene
Description: Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).
UOM: 1 * 10 µG
Product Image-70556-3

Expression vectors, pET-41 DNA, Novagen®

Supplier: Merck Millipore (Novagen)
Description: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Product Image-69750-3

pET-24b(+) DNA, Novagen®

Catalog Number: (69750-3)
Supplier: Merck Millipore (Novagen)
Description: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).
UOM: 1 * 10 µG
Image Unavailable-70595-3

pSTBlue-1 AccepTor™ vector kit

Supplier: Merck Millipore (Novagen)
Description: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

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Expression vectors, pGEX

Supplier: Cytiva
Description: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Product Image-27-1542-01

E. coli BL21

Catalog Number: (27-1542-01)
Supplier: Cytiva
Description: The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
UOM: 1 * 1 Vial
Product Image-PIER16191

Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors

Supplier: Thermo Fisher Scientific
Description: <i>Gaussia</i> Dura Luc vectors contain a mutant form of the <i>Gaussia</i> luciferase gene that confers better bioluminescent signal stability than native luciferase. <i>Gaussia</i>-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Product Image-27-1524-01

M13KO7 Helper Phage, Cytiva

Catalog Number: (27-1524-01)
Supplier: Cytiva
Description: M13 phage for the GST expression system.
UOM: 1 * 100 µl
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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