You Searched For: Protein Purification

Catalog Number: (61768-1ML)

Supplier: Merck

Description: 50% aqueous suspension in 1 M NaCl.

UOM: 1 * 1 mL

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Supplier: Avantor

Description: The J.T.Baker^® BAKERBOND^® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

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Supplier: Avantor

Description: J.T.Baker® BAKERBOND® protein precipitation plates enable fast protein removal from biological samples.

Supplier: Avantor

Description: J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

Catalog Number: (1.04765.0500)

Supplier: Merck

Description: Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.

UOM: 1 * 500 g

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Supplier: G-Biosciences

Description: Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Supplier: Avantor

Description: Silica-based media used for the purification of proteins based on differential salt induced hydrophobicity. Mixed mode provides unique selectivity with its hydrophobic interaction and ion exchange function.

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Catalog Number: (IMMRIR1011)

Supplier: ImmunoReagents

Description: ImmunoReagents Human IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Human IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

UOM: 1 * 2 mL

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Catalog Number: (IMMRIR1012)

Supplier: ImmunoReagents

Description: ImmunoReagents Llama IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Llama IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

UOM: 1 * 2 mL

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Catalog Number: (786-789)

Supplier: G-Biosciences

Description: Immobilised Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin.

UOM: 1 * 5 mL

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Supplier: G-Biosciences

Description: Jacalin, or <i>Artocarpus integrifolia</i> lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (<i>Artocarpus integrifolia</i>) seeds.

Supplier: Rockland Immunochemicals

Description: Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.

Supplier: Genscript

Description: Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

Catalog Number: (786-802)

Supplier: G-Biosciences

Description: The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Catalog Number: (786-803)

Supplier: G-Biosciences

Description: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

UOM: 1 * 1 KIT

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