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You Searched For: Protein Linking and Labeling Systems

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.


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Product Image-M336-1L

VWR® Antibody signal enhancer

Catalog Number: (M336-1L)
Supplier: VWR Chemicals
Description: Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.
UOM: 1 * 1 L
Product Image-92260.

Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Supplier: Biotium
Description: Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.

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Product Image-786-383

femtoCHROMO™- AP Substrate for chromogenic detection

Supplier: G-Biosciences
Description: A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.

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CF™ dye bovine serum albumin conjugates

Supplier: Biotium
Description: Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.

Image Unavailable-APOSBIL3122-250ML

SeramunBlock

Supplier: Apollo Scientific
Description: Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.

Image Unavailable-APOSBIL3117-100ML

AP-conjugate stabiliser, SeramunStab® STAP2

Catalog Number: (APOSBIL3117-100ML)
Supplier: Apollo Scientific
Description: Liquid product red odourless pH-value 7,5 to 7,7. Stabiliser for a broad range of AP conjugates for various assay conditions. Useful for antibodies sensitive to pH <7,0.
UOM: 1 * 100 mL
Image Unavailable-ENZOALX620072M001

ZnAF-2.tetrahydrochloride

Catalog Number: (ENZOALX620072M001)
Supplier: ENZO LIFE SCIENCES
Description: Fluorescent probe for Zn2+
UOM: 1 * 1 mg

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Image Unavailable-786-694

Biotin kits, Micro HOOK™

Supplier: G-Biosciences
Description: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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Dye conjugates dUTP CF®

Supplier: Biotium
Description: CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for <i>in situ</i> hybridisation and nucleic acid blotting applications.

Image Unavailable-80500.

Reactive quencher dyes

Supplier: Biotium
Description: Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

Image Unavailable-CAYM17170-100

Bilirubin conjugate, sodium salt

Supplier: Cayman Chemical
Description: Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

Image Unavailable-COBSD4-000

B-Phycoerythrin (BPE)

Catalog Number: (COBSD4-000)
Supplier: Columbia Biosciences
Description: Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
UOM: 1 * 1 mg
Image Unavailable-92224.

Biotin SE protein labelling kit

Catalog Number: (92224.)
Supplier: Biotium
Description: This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
UOM: 1 * 1 KIT
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Reactive oxygen species indicators dyes

Supplier: Biotium
Description: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

MSDS

Image Unavailable-ENZOALX850023KI01

Phosphoserine detection set

Catalog Number: (ENZOALX850023KI01)
Supplier: ENZO LIFE SCIENCES
Description: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.
UOM: 1 * 1 SET

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Image Unavailable-APOSBIL3113-100ML

HRP-conjugate stabiliser, SeramunStab® STNP

Catalog Number: (APOSBIL3113-100ML)
Supplier: Apollo Scientific
Description: Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.
UOM: 1 * 100 mL
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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